Kinetics of In Sacco Fiber-Attachment of Representative Ruminal Cellulolytic Bacteria Monitored by Competitive PCR
نویسندگان
چکیده
منابع مشابه
Differential fermentation of cellulose allomorphs by ruminal cellulolytic bacteria.
In addition to its usual native crystalline form (cellulose I), cellulose can exist in a variety of alternative crystalline forms (allomorphs) which differ in their unit cell dimensions, chain packing schemes, and hydrogen bonding relationships. We prepared, by various chemical treatments, four different alternative allomorphs, along with an amorphous (noncrystalline) cellulose which retained i...
متن کاملUtilization of individual cellodextrins by three predominant ruminal cellulolytic bacteria.
Growth of the ruminal bacteria Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1, and R. albus 7 followed Monod kinetics with respect to concentrations of individual pure cellodextrins (cellobiose, cellotriose, cellotetraose, cellopentaose, and cellohexaose). Under the conditions tested, R. flavefaciens FD-1 possesses the greatest capacity to compete for low concentrations of these c...
متن کاملCompetition among three predominant ruminal cellulolytic bacteria in the absence or presence of non-cellulolytic bacteria.
Competition among three species of ruminal cellulolytic bacteria - Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1 and Ruminococcus albus 7 - was studied in the presence or absence of the non-cellulolytic ruminal bacteria Selenomonas ruminantium or Streptococcus bovis. Co-cultures were grown under either batch or continuous conditions and populations were estimated using species-sp...
متن کاملLocalization of ruminal cellulolytic bacteria on plant fibrous materials as determined by fluorescence in situ hybridization and real-time PCR.
To visualize and localize specific bacteria associated with plant materials, a new fluorescence in situ hybridization (FISH) protocol was established. By using this protocol, we successfully minimized the autofluorescence of orchard grass hay and detected rumen bacteria attached to the hay under a fluorescence microscope. Real-time PCR assays were also employed to quantitatively monitor the rep...
متن کاملAdhesion of cellulolytic ruminal bacteria to barley straw.
Adhesion of the cellulolytic ruminal bacteria Ruminococcus flavefaciens and Fibrobacter succinogenes to barley straw was measured by incubating bacterial suspensions with hammer-milled straw for 30 min, filtering the mixtures through sintered glass filters, and measuring the optical densities of the filtrates. Maximum adhesion of both species occurred at pH 6.0 and during mid- to late-exponenti...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 2003
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(03)73726-6